Metagenomic profiling dataset of bacterial communities of a drinking water supply system.pdf (217.36 kB)
Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
journal contributionposted on 2019-08-29, 10:49 authored by Innocentia Guguletho ErdoganInnocentia Guguletho Erdogan, Lukhanyo Mekuto, Prof Seteno Karabo Obed NtwampeProf Seteno Karabo Obed Ntwampe, Elvis Fosso-KankeuElvis Fosso-Kankeu, Frans B. Waanders
The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence
North-West University, the Cape Peninsula University of Technology University Research Fund (Grant no. URF RY12) and the National Research Foundation of South Africa.