The role of gold nanoparticles synthesized using extracts of Cyclopia intermedia in the anticancer efficacy of Doxorubicin.

The first part of the study investigated and compared the phytochemical, total polyphenolic contents (TPC), and antioxidant capacity of fermented HB (FHB) and green/unfermented HB (GHB) plant to determine if these extracts have the reducing capacity to produce biogenic AuNPs. The result revealed that both the GHB and FHB plants exhibited significant phytochemical and antioxidant contents. Further, the phytochemical and antioxidant contents of GHB and FHB plants were leveraged for the reduction gold salt for the synthesis of gold nanoparticles (AuNPs). While the synthesis of AuNPs from MGF was done following published protocol, the successful synthesis of AuNPs from the GHB and FHB plant extracts begins with the optimization of synthetic parameters such as temperature, reaction time, and concentration of plant extract was achieved.