Cape Peninsula University of Technology

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The in vitro modulation of intestinal growth and inflammatory Indices by Fumonisin B1 and hydrolyzed Fumonisin B1 through protein network analyses

posted on 2024-04-25, 12:43 authored by Nabeela GamietNabeela Gamiet

The intestinal epithelium is regularly exposed to contaminants like fumonisin mycotoxins which are associated with mycotoxicosis in various mammalian species. Fumonisin B1 (FB1) can also enzymatically convert to hydrolyzed fumonisin B1 (HFB1), a less potent ceramide synthase inhibitor, with contradictory outcomes in diverse in vitro and in vivo models. In this study, we evaluated the impact of FB1 and HFB1 on cell viability, apoptosis, and proliferation in the porcine intestinal cell line (IPEC-J2), while also monitoring inflammatory responses through interleukin 8 (IL-8) immune-detection. The molecular mechanisms and pathways influenced by FB1 and HFB1 exposure were further investigated through proteomic and bioinformatic analyses. Using Gene Ontology (GO), Differentially Abundant Proteins (DAPs) were identified and visualized by comparing Homo sapiens and porcine databases and 52 significant DAPs were found between FB1 and HFB1 in comparison to the control. Fibronectin 1 (FN1), an adhesive glycoprotein of the intestine, was consistently detected in cells exposed to FB1 and HFB1. FB1 up-regulates FN1, while HFB1 down-regulates it, leading to distinct cancer-promoting pathways as revealed by Cytoscape and STRING enrichment analysis. These results suggest that HFB1 promotes a greater in vitro toxicity upon the IPEC-J2 cell line compared to FB1, due to the abundance of proteins that were affected during exposure and the interconnectedness of pathways that were enriched.


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