Metagenomic data of free cyanide and thiocyanate degrading bacterial communities
Version 2 2019-10-28, 14:18Version 2 2019-10-28, 14:18
Version 1 2017-12-01, 13:37Version 1 2017-12-01, 13:37
dataset
posted on 2019-10-28, 14:18authored byMekuto, Lukhanyo Ntwampe, Seteno KO Mudumbi, John BN Akinpelu, Enoch A Mewa-Ngongang, Maxwell
The data presented in this article contains the bacterial community
structure of the free cyanide (CN-
) and thiocyanate (SCN-
)
degrading organisms that were isolated from electroplating wastewater
and synthetic SCN- containing wastewater. PCR amplifi-
cation of the 16S rRNA V1-V3 regions was undertaken using the
27F and 518R oligonucleotide primers following the metacommunity
DNA extraction procedure. The PCR amplicons were
processed using the illuminas reaction kits as per manufacturer's
instruction and sequenced using the illuminas MiSeq-2000, using
the MiSeq V3 kit. The data was processed using bioinformatics
tools such as QIIME and the raw sequence files are available via
NCBI's Sequence Read Archive (SRA) database.
& 2017 Published by Elsevier Inc. This is an open access article
under the CC BY license