Assessment of the relationship between adiposity, Sirtuin 1, and miR-30a-5p in a mixed-ancestry population in Bellville South, Cape Town.

CPUT/HWS-REC 2024/H1 (Renewal)

This dataset contains raw and processed data from a study investigating the expression of SIRT1 and miR-30a-5p across different BMI categories in a mixed-ancestry population from Bellville South and Belhar, Cape Town, South Africa. The dataset includes ELISA absorbance readings for SIRT1 protein quantification as well as qPCR data for miR-30a-5p expression analysis. Key variables include sample IDs, absorbance values, cycle threshold (CT) values, ΔCT, 2^-ΔCT, ΔΔCT, and 2^-ΔΔCT calculations. The dataset was used to explore the relationship between these biomarkers and cardiometabolic health indicators.

Study Results: A total of 150 females and 150 males were selected for this study with an average age of 50 years. miR-30a-5p expression was significantly higher in overweight and obese participants compared to normal-weight individuals. SIRT1 protein concentration decreased with obesity, consistent with miR-30a-5p-mediated repression, while SIRT1 gene expression was increased in obese and high WC groups, suggesting post-transcriptional regulation of SIRT1 by miR-30a-5p.

Conclusion: These findings contribute to understanding the relationship between miR-30a-5p and SIRT1 in obesity and their potential as biomarkers and therapeutic targets for metabolic regulation. Insights into metabolic regulatory mechanisms contribute to the identification of therapeutic pathways in populations with high obesity prevalence, such as South Africa’s mixed-ancestry community.